The formulation of gel depends upon factors like temperature modulation, ph changes, presence of ions and ultraviolet irradiation, from which drug gets released in. Stability, acta pharmaceutica hungarica 78 2008 2330 if. Tinidazole periodontal gel was prepared by different concentrations of gellan gum, poloxamer 407. Formulation development and evaluation of thermoreversible.
Development of valproic acid niosomal in situ nasal gel. In situ forming polymeric formulations drug delivery systems is in sol form before administration in the body, but once administered, undergoes gelation in situ to form a gel, from which drug gets released in sustained and controlled manner. Three in situ polymer gel systems including bulk gels, colloidal dispersion gels, and weak gels are often used to mitigate water production caused by reservoir heterogeneity and unfavorable mobility ratio of oil and injected fluids. Extraction and evaluation of tamarind seed polysaccharide as. Heat the tamarind seed polysaccharide polymer and sodium alginate solution at 6070. Formulation and evaluation of an in situ gelforming.
The temperature sensitive in situ gel formulations, undergo phase transition from liquid to semisolid gel upon exposure to physiological eye temperature. Formulation and evaluation of an in situ gelforming ophthalmic. Selecting the most appropriate gel system is a key component for a successful conformance improvement treatment. The prepared insitu gel was evaluated for ph, gelation temperature, drug content, mucoadhesive force, gel strength and viscosity measurement. Three insitu polymer gel systems including bulk gels, colloidal dispersion gels, and weak gels are often used to mitigate water production caused by reservoir heterogeneity and unfavorable mobility ratio of oil and injected fluids. On heating a gellan sample in an ionfree medium, the polymer. Hec ds gel exhibited significantly better drug release when compared to commercial gel. The aim of this thesis is the development and characterization of controlled release formulations based on thermo. The graduate school, silpakorn university has approved and accredited the thesis title of preparation of in situ forming gel systems for delivery of antimicrobial agents for pe. Benefiting from the merits of both a solution and a gel, an impressive number of in situ gel forming systems induced by temperature, ph and ions have been prepared for use in nasal drug delivery in the past few years. Two new methods were developed, one more cost effective proximity assay without the use of enzymes and one. The formulation of gel depends upon factors like temperature modulation, ph changes, presence of ions and ultraviolet irradiation.
Development and characterization of insitu gel for. Formulation and investigation of in situ gelling lyotropic. A thiolene photoclick polymerization 4 wt% peg4nbdtt. In situ gel drug delivery systems are used in sol form before administration in the body, but once administered, undergo gelation in situ, to form a gel. The ph of in situ gel solution was found to be around 6. Then, the gel is laid on 2% agar gel 5 mm thick containing 0. The main objective of present study is to formulate and evaluate methyl cellulose based in situ periodontal gel of atorvastatin. Telechelic polymers with hydrophilic midblocks polyethylene glycol, peg and hydrophobic end groups fluoroalkyl, rf are synthesized and explored as candidates for in situ forming hydrogels for biomedical applications. Thermosensitive in situ gel for ocular delivery of lomefloxacin. Review article asian journal of biomedical and pharmaceutical sciences 1 4 2011, 0107 corresponding author. They undergo in situ phase transition to form a strong gel that is capable of withstanding shear forces in the culdesac and of. About 1 ml of sample was withdrawn at a time interval of 1 hour and replaced with. Sz ucs maria, budai szabolcs, er os istvan, gelemulsion systems i. Insitu gel formation for ocular drug delivery system an.
Graduate school etd form 9 purdue university graduate school. A weighed amount of poloxamer 407 and poloxamer 188 1520% wv was slowly added to 15 ml water at c in a beaker with continuous stirring using a magnetic stirrer at a speed of 500 rpm for 2 hrs. Polymer gels as pharmaceutical dosage forms diva portal. In situ hybridization permits visualization of a specific cell population in a whole embryo without this masking effect, as seen in figures 24 above. For the insitu nasal gel formulations of montelukast sodium, it was observed that the invitro permeation profiles of all the different formulations of insitu gels obeys higuchi model for formulations f3 and f4 and zero order for formulations f5 and f6. Controlled release ion sensitive floating oral in situ gel. Thus, factorial designs were applied to optimize the formulation and development of floating matrix tablet, in situ gel and mucoadhesive beads. Alkaline membrane fuel cells with insitu crosslinked ionomers yongjun lenga, lizhu wangb, michael a. The present work concerns with the formulation, evaluation and optimization of floating oral in situ gel of itopride hydrochloride for controlled release. Formulation of gels depends on factors like temperature modulation, ph change, presence of ions and ultra violet irradiation, from which the drug gets released in a sustained and controlled manner.
Formulation and evaluation of periodontal in situ gel. The in situ gel was prepared by using different concentration of methyl cellulose and gel was. Thermosensitive in situ gel for ocular delivery of. In situ forming polymeric formulations are drug delivery systems that are in sol form before administration in the body, but once administered, undergo gelation in situ, to form a gel. Mason, marine estuarine environmental science dissimilative sulfate reduction is a major source of sulfide in sediments and the water colu mn of lakes and estuaries.
They undergo in situ phase transition to form a strong gel that is capable of withstanding shear forces in the culdesac and of sustaining drug release at physiological conditions. Thus the in vitro dissolution test indicated the sustained release nature of in situ gel of ciprofloxacin hydrochloride. So, gelation temperature of poloxamer 188 in situ gel was not fulfilling the requirement of in situ gel formulation. Mechanisms and kinetics of gel formation in geopolymers. Thesis formulation and investigation of gelemulsions. Attenuated total reflectance fourier transform infrared spectroscopy atrftir is used to analyse partially reacted geopolymers and hardened pastes both in and ex situ. Evaluation of a new liquid breaker for polymer based insitu gelled acids. A gel probe sampler has been constructed to study in situ reductive dissolution of mn oxides.
Gelation temperature and time of atorvastatin drug release kinetics in situ gel to understand the drug release kinetics of atorvas methyl cellulose gelation gelation time tatin in situ gel formulation. A thesis submitted to the faculty of purdue university by han shih. Selected dependent variables were concentration of gellan. After the electrophoresis, the gel is washed twice with 25% 2. Formulation and evaluation of floating sustained release in. These have a characteristic property of temperature dependent, ph dependent and cation induced. Budai szabolcs, sz ucs maria, er os istvan, gelemulsion systems ii. Parameswari, p 20 studies on temperature induced mucoadhesive in situ gel formulation of rizatriptan benzoate for nasal administration. Formulation and evaluation of nasal in situ gel for. Benefiting from the merits of both a solution and a gel, an impressive number of in situ gelforming systems induced by temperature, ph and ions have been prepared for use in nasal drug delivery in the past few years. In situ forming hydrogels for drug delivery to the. Polymer gel systems as water management materials have been widely used in recent years for enhanced oil recovery applications. Percentage drug release in case of in situ gel of ciprofloxacin hydrochloride was found to be 67.
In situ forming polymeric formulations drug delivery systems is in sol form before administration in the body, but once administered, undergoes gelation in situ to form a gel. These are freeflowing liquid at room temperature and easy to administer into the eye as drops. A total of 40 in situ forming hydrogels were prepared and stability tests were carried out over 3 months, in some cases up to 23 months at 4 c, 25 c and 40 c. The main aim of this work was to formulate an in situ forming hydrogel containing a subantimicrobial dose of doxycycline that gels at physiological conditions with. Extraction and evaluation of tamarind seed polysaccharide. Next, i have been blessed to work so closely with such a wonderful research group, dr. Thermally gelling insitu gel formulations for nasal administration were prepared by using polymers such as poloxamer 407 and hpmc k4m in varying concentrations by using cold method. So, 1520% wv concentration of poloxamer 407 was used for further studies. Formulation development and evaluation of thermoreversible mucoadhesive in situ nasal gel of asenapine maleate volume.
Gelling capacity, in situ gel, in vitro diffusion study, moxifloxacin. Effects of nicotine on embryological neural development a. Anti alzheimers drug, in situ gel, nasal drug absorption, invitro diffusion study. The amount of mn released from the gel by reductive. Mahak shaikh, aarti mandloi, vishnu yadav, gopkumar p. Attenuated total reflectance fourier transform infrared spectroscopy atrftir is used to analyse partially reacted geopolymers and hardened pastes both in. In vitro drug release studies in vitro release study of in situ gel solution is carried out by using franz diffusion cell. The aim of this thesis is the development and characterization of controlled release formulations based. Ag 2s was formed preferentially at the exterior surface of the gel and was consequently prone to becoming detached with handling. This thesis is the result of work carried out at the department of chemistry. Although this produced a very even and finely dispersed binding gel, sulfide measurements were irreproducible. The gel is equilibrated with 10 mm sodium phosphate buffer ph 7. Formulation and evaluation of in situ gels containing.
In situ gel forming systems are viscous liquids that shift to a gel phase upon exposure to physiological conditions. The gel consists of a polyacrylamide polymer matrix doped with hydrous mn oxide hmo. Advances in the modelling of insitu powder diffraction data. Controlled release ion sensitive floating oral in situ gel of. Gel slabs are mounted into a probe, which is designed to be inserted into the sediments. After 24 hours the oil was added to this gel while the sample was stirred. The gastropod cellulase is mixed with an equal volume of the loading buffer comprising 20% glycerol, 0. In situ gels of clotrimazole for oral candidiasis gel dosage forms are successfully used as drug delivery systems to control drug release and protect the medicaments from a hostile environment.
Lomefloxacin hydrochloride is a broad spectrum, second generation fluroquinolone antibiotic used to treat various conjunctival infections. Preparation of in situ gelling delivery system 79 4. Further insitu gel was evaluated for its in vitro drug diffusion study. These in situ solutions are liquid at room temperature but undergo gelation when in contact with body fluids or change in ph. The aim of this study was to formulate niosomal insitunasal gel of valproic acid for lowering the dosage, improving patient compliance and to provide prolonged action. In this research work an attempt was made to formulate and evaluate insitu gel for the intranasal delivery of montelukast sodium for the effective treatment of asthma. By using the niosomal insitu nasal gel formulation, an attempt was made to increase its permeation so as to increase its bioavailability. Between donor and receptor compartment dialysis membrane is placed 0. The main objective is to formulate and evaluate in situ oral topical gels of clotrimazole based on the concept of ph triggered and ion activated systems.
Budai szabolcs, sz ucs maria, er os istvan, gel emulsion systems ii. Studies on temperature induced mucoadhesive in situ gel. Different concentrations of polymer were used to prepare in situ gel as per the composition shown in table 1. Physicalchemical characterisation, acta pharmaceutica hungarica 78 2008 1121 if. In this thesis applications of the in situ proximity ligation assay in situ pla were developed for diagnostics and research. Pdf in situ gelling systems for drug delivery researchgate. In situ gels are systems which are applied as solutions or suspensions and are capable of undergoing rapid soltogel transformation triggered by external stimulus such as temperature, ph etc. Alkaline membrane fuel cells with insitu crosslinked. Only 15% wv and 20% wv concentration of poloxamer 407 in situ gel showed ability to form gel in the range of body temperature shown in table 3. The formulation placed in donor compartment and freshly prepared simulated tear fluid in receptor compartment.
Spectroscopic ellipsometry was utilized to follow in situ the annealing of sol. Journal of pharmacy and pharmaceutical sciences formulation and evaluation of floatable insitu gel for stomachspecific drug delivery of vanlafaxine hcl. The formation of gels depends on factors like temperature modulation, ph change, presence of ions and ultra violet irradiation, from which the drug gets released in a sustained and controlled manner. Formulation and evaluation of floating sustained release. Chapter 3 a hydrous manganese oxide doped gel probe sampler.
Gry institute of pharmacy, vidya vihar, borawan, khargone, madhya pradesh 451228, india. Ph sensitive polymers like carbomers form gel upon change in their environment ph. In situ forming hydrogels using selfassembly of fluoroalkyl. Hydrophilic gel, hydroxyethylcellulose, diclofenac sodium, in vitro drug release. Isl1 expression was slightly reduced in specific cranial nerves, as seen through in situ hybridization, most noticeably the vagus nerve in nicotinetreated embryos. However, most polymer gel systems are limited in their ability to withstand the harsh environments of high temperature and high salinity. Relevant physical properties, including phase behavior, rheology and erosion kinetics, are characterized to guide rational design of polymers for specific applications. Applications of in situ proximity ligation assays for. Pdf on aug 1, 2014, maryam kouchak and others published in situ gelling. Insitu forming polymeric formulations drug delivery systems is in sol form before administration in the body, but once administered, undergoes gelation insitu to form a gel, from which drug gets released in sustained and controlled manner. All the four formulations were prepared as described below. In situ, highresolution measurement of dissolved sulfide. Carbopol 934p is a polyacrylic acid, which undergoes soltogel transition in aqueous solution when the ph is raised above its pka value of about 5. The objective of the present study was to formulate and evaluate a gastro retentive in situ gelling system of ciprofloxacin hcl using hpmc k100m, sodium alginate gelling polymer, calcium chloride and calcium carbonate as a cross linking agent for.
Mainly in situ gels are administered by oral, ocular. The formation of gel depends on factors like temperature modulation, ph change, presence of ions and ultraviolet irradiation, electrical. The in situ gelling polymer gellan gum was found to have a favourable rheological and in. This is to certify that the thesis entitled studies on in situ gelling system for better. In situ measurement of sulfide in natural waters michael sean rearick, master of science, 2004 thesis directed by. Insitu forming polymeric formulations drug delivery systems is in sol form before administration in the body, but once administered, undergoes gelation insitu to form a gel. Hicknerb, chaoyang wanga,b, aelectrochemical engine center, department of mechanical and nuclear engineering, the pennsylvaniastate university, university park, 16802. Mmps with subantimicrobial dose of topical doxycycline containing gel, it was shown that ulcers healed completely by day 3 in majority of patients. Photopolymerizable gels are formed in situ but they are. Insitu gel formation for ocular drug delivery system an overview. Insitu gel drug delivery system linkedin slideshare. Maliba pharmacy college, maliba campus, gopal vidyanagar, uka tarsadia university, bardolimahuva road, tarsadi, surat 394350, gujarat, india. Effects of nicotine on embryological neural development a senior honors thesis. Masters thesis, adhiparasakthi college of pharmacy, melmaruvathur,tamil nadu, india.
The n values from the drug release experiment for the formulations f3, f4, f5 and f6. The aim of the present study was to formulate and evaluate ph responsive insitu gel for ophthalmic delivery. Preparation and evaluation of novel in situ gels containing. In situ forming nasal drug delivery systems gellan gum and xanthan gum were used as in situ gel forming polymers animal studies were conducted using an allergic rhinitis model and the effect of in situ gel on antigen induced nasal symptoms in sensitized rats was observed insitu gel was found to inhibit the increase in nasal symptoms as compared to marketed formulation nasonex mometasone furoate suspension 0. The gel phase of sol gel coexisting polymers can be transformed into an injectable state by the addition of a biotolerable organic solvent, such as nmethyl pyrrolidone nmp, and this solution can be restored to a hydrogel state quickly after injection by removal of the organic solvent by diffusion. The first method of preparing the binding gel was therefore adopted. C addition of trisodium citrate in above solution decrease the solution.
Sz ucs maria, budai szabolcs, er os istvan, gel emulsion systems i. The formulation should have an optimum viscosity that will allow for easy instillation into the eye, which would undergo a rapid sol to gel transition triggered by ion exchange as shown in table 2. The in situ gel formulations were placed in the sampler tube. To view anatomical expression patterns, in situ hybridization was used to compare control and nicotine treated embryos of the same ages. Hec can be used as gelling agent for the development of gel formulations, because of its good release profile, watersoluble nature and good spreadability. Yangyao lee for the synthesis work of the tio 2 flakes, dr. Approaches ofin situ gel drug delivery there are four broadly defined mechanisms used for triggering thein situ gel formation of biomaterials. Ciprofloxacin hydrochloride is popularly used as a broad spectrum antibiotic. Composition of drug and excipients in factorial batches of periodontal in situ gel of. Chapter 3 a hydrous manganese oxide doped gel probe. Formulation and evaluation of floating in situ gel based. This thesis concentrates on gel formulations intended for mucosal drug delivery. Controlled release gel formulations and preclinical screening of drug candidates abstract simple gel formulations may be applied to enhance the systemic and local exposure of potential compounds. Comprehensive guidelines for the application of insitu.